University of Pretoria
Browse
Dissertation Supplementary Data.pdf (4.23 MB)

Analysis of two triterpenes (ARM-2 and RA-5) from Protorhus longifolia

Download (4.23 MB)
dataset
posted on 2024-05-16, 12:54 authored by Naleli RamasiaNaleli Ramasia

The data obtained in Appendix A is the in silico studies carried out on the triterpenes. Images of all compounds that were docked against some proteins and enzymes (CD-36, SR-A1, ABCA-1, ABCG-1, LOX-1, ACAT-1 and sPLA2) involved in cholesterol trafficking, is provided. Molecular docking data obtained shows the docking score and glide energy of each compound. Ligand-receptor interactions are provided to indicate how the ligand receptor complex is potentially formed and supports the docking score. All possible ADMET properties, drug-likeness, and SwissTarget predictions was predicted for the compounds (ARM-2, RA-5 and RA-3). Appendix B presented in the document describes supplementary data from in vitro studies. The standard curve for malondialdehyde (MDA) used in the TBARS studies to determine the amount of MDA inhibited. This was followed by data that shows the qualitative and quantitative analysis of the effects of ARM-2 and RA-5 on ox-LDL induced lipid accumulation in RAW264.7 macrophage cells. The images of oil-red O-stained lipid droplets (foam cell formation) are provided. The images show lipid droplet formation in an atherogenic environment in the presence or absence of the reference drug (lovastatin), ARM-2 and RA-5. The percentage lipid content present in the cells was determined in cells stained with oil-red O and nile red.

Funding

University of Pretoria (UP) Postgraduate Bursary

National Research Foundation (NRF)

History

Department/Unit

Biochemistry, Genetics and Microbiology

Sustainable Development Goals

  • 3 Good Health and Well-Being