University of Pretoria

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Reason: Data for this study is under embargo due to the researchers still in the process of publishing the data included herewith.





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Angiogenic, apoptotic and autophagic profiling of chronic myeloid leukaemia patients’ platelets ex vivo before and after treatment with Imatinib

posted on 2022-02-10, 09:48 authored by Lisa RepsoldLisa Repsold, Annie Margaretha Joubert, Gregory R. Tintinger

The data presented here was to determine the angiogenic, apoptotic, and autophagic profiles of chronic myeloid leukaemia patients ex vivo on platelets before and after treatment with Imatinib. Platelet counts, viability and activation were determined by human cluster of differentiation (CD) 41 and -62 markers. The possible occurrence of apoptosis and autophagy was measured by means of flow cytometry and western blot analysis via Annexin V-fluorescein isothiocyanate (FITC), caspase 3, autophagy related gene 5 (Atg5) and light chain I and II (LC3) markers. Angiogenesis expression was determined through vascular endothelial growth factor (VEGF) and transforming growth factor β (TGFβ) markers.


National Research Foundation (N00591, N00375)

Struwig-Germeshuysen Research Trust (AON074)

Medical Research Council of South Africa (AOW110)

Cancer Association of South Africa (AOW228, AOV741)

School of Medicine Research Committee of the Faculty of Health Sciences at the University of Pretoria (AOR984)