Angiogenic, apoptotic and autophagic profiling of chronic myeloid leukaemia patients’ platelets ex vivo before and after treatment with Imatinib

The data presented here was to determine the angiogenic, apoptotic, and autophagic profiles of chronic myeloid leukaemia patients ex vivo on platelets before and after treatment with Imatinib. Platelet counts, viability and activation were determined by human cluster of differentiation (CD) 41 and -62 markers. The possible occurrence of apoptosis and autophagy was measured by means of flow cytometry and western blot analysis via Annexin V-fluorescein isothiocyanate (FITC), caspase 3, autophagy related gene 5 (Atg5) and light chain I and II (LC3) markers. Angiogenesis expression was determined through vascular endothelial growth factor (VEGF) and transforming growth factor β (TGFβ) markers.