Antibiotic resistance, production of extended-spectrum beta-lactamases (ESBLs), carbapenemases, and molecular epidemiology of Klebsiella pneumoniae

Characterisation and investigation of the molecular epidemiology and beta-lactam resistance of invasive K. pneumoniae isolates from a private laboratory in Gauteng, South Africa. This included antibiotic susceptibility profiles for isolates, confirmation of phenotypic production of ESBL, AmpC and carbapenemase, and detection of specific resistance genes. Genotyping of isolates was conducted using pulsed field gel electrophoresis and subsequently a dendrogram was constructed using the  pulsed field gel electrophoresis (PFGE) profiles of each isolate to identify pulsotypes. Genomic investigation was conducted on selected representative isolates using Next Generation Sequencing. Bioinformatics analysis was conducted on the NGS data.