Construction of CRISPR/Cas9 BBTV vectors and evaluation of their gene editing efficiency in banana for resistance to banana bunchy top virus (BBTV)

The primary objective of this study was to construct CRISPR/Cas9 vectors targeting the Dynamin-Related Protein 1C (DRP1C) gene and the master replication gene of Banana Bunchy Top Virus (BBTV DNA-R), and to evaluate their gene-editing efficacy in Nicotiana benthamiana and banana protoplasts. The dataset includes gel electrophoresis images, photographs of BBTV-infected plants, visualizations of the CRISPR/Cas9 mechanism, and comprehensive tables summarizing experimental data. It also contains all primer sequences used for designing single-guide RNAs (sgRNAs), details of modular Golden Gate cloning for DRP1C and BBTV DNA-R CRISPR/Cas9 vectors, sgRNA screening results, and information on plasmid constructs and sequences used in cloning. Additionally, the dataset includes mutation frequency data from banana protoplast assays, gel images confirming successful cloning, sequencing results, and full compositions of all media and buffer solutions used in the study.