The research presented in aimed to determine the taxonomic position of Bradyrhizobium species isolated from the root nodules of Acacia dealbata, Acacia decurrens and Acacia mearnsii in a previous study. Additionally, we aimed to increase the knowledge of Bradyrhizobium diversity occurring in the root nodules of A. mearnsii, A. dealbata and A. decurrens growing in invasive settings. This was achieved through phenotypic analysis, genealogical concordance analysis and Multilocus sequence analysis of various housekeeping genes. While, phylogeographic inferences regarding the symbiotic properties were made based on phylogenetic analysis of sequences of the common nodulation gene nodA.