Fruit flies sampled from the South African highveld and lowveld for the assessment of fruit fly assemblages and Wolbachia presence

<p>The datasets uploaded in this collection include:</p> <p>1. R script for nmds (nmds_NK_R.R) along with the csv files used (data and design).</p> <p>2. Two molecular datasets for the extractions.</p> <p>3. Fruit fly collection data for the highveld and the lowveld.</p> <p>The project aimed to assess the viability of a <em>Wolbachia</em>- driven insect incompatibility technique control method as a means to aid farmers with the challenges posed by fruit flies. This was done through the completion of the following objectives:</p> <p>1. Establish the species diversity of tephritid flies in two fruit producing regions of South Africa, the highveld and lowveld, based on morphological identification.</p> <p>2. Assess the intraspecific genetic diversity of tephritid flies from the highveld and lowveld using a mitochondrial cytochrome oxidase 1 gene barcoding approach.</p> <p>3. Determine the prevalence and diversity of <em>Wolbachia</em> in naturally infected tephritid species from the highveld and lowveld using PCR-sequencing and next generation sequencing.</p> <p><br></p>