University of Pretoria

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Geographical distribution of marama bean associated with fungal species and the investigation of the fungal diversity of marama bean

posted on 2022-10-19, 13:35 authored by Taryn ArmfieldTaryn Armfield, Nigel P. Barker, Quenton Kritzinger, Mariette Truter

Data include figures, tables and protocols of the study.

Figures 1 and 2 pertain to the geographical distribution of the marama bean (Tylosema esculentum and T. fassoglense), throughout southern Africa, according to herbarium specimens.

Table 1 provides information regarding common known mycotoxins, there fungal producers, plant host, and known human, animal and plant toxicity. As well as the references pertaining to the table.

Protocols 1-5 provide the molecular identification protocols followed during the study, including the Zymo Manufacturers guidelines for deoxyribonucleic acid (DNA) extraction, the quantities and cycling conditions requred for DNA amplification, cleanup and sequencing, as well as the protocol for sodium acetate-ethanol precipitation required before submitting samples for analysis.

Table 2 provides the genus/species names of the fungal isolates isolated during the study, as well as the closest similarity match according to the NCBI GenBank and MycoBank databases, using the Internal Transcribed Spacer (ITS) region and Beta-tubulin (BenA) or the Translation elongation factor 1-α (TEF-1α) gene regions.

Table 3 contains information related to all of the fungal DNA reference strains associated with the Aspergillus, Penicillium and Fusarium species isolated, used for the phylogenetic analysis of the three fungal genera.

Table 4 indicates the results of detached leaf assays performed (images of the leaves), in which marama bean leaves were inoculated with various fungal species isolates and incubated to determine the pathogenic potential of fungal species. 

Table 5 provides a visual representation of the detached leaf assay, focusing on the number of plates/leaves per sample (total 3 per sample) that indicated disease symptoms related to the specific fungal species used to inoculate the leaves.

Tables 6 and 7 provide the individual and average disease ratings per week per trial, over a six and five week period for Trials 1 and 2 respectively. A scoring system (0-20) was created to determine disease ratings across different symptoms produced by different fungal species.

Tables 8 to 12 contain the above ground (stems and leaves) and below ground (roots and tubers), wet and dry weight measurements (grams) of the marama bean seedlings per fungal species' incoulated treatment after the pathogencity trials concluded, indicating the individual and average seedling weights. 


Department of Science and Technology (DST)/National Research Foundation (NRF)

South African Research Chairs Initiative (SARChl) in the National Development Plan Priority Area of Nutrition and Food Security, Unique number: SARCI170808259212



Plant and Soil Sciences