The dataset is for a study titled "Insights into the healthcare workers' perceptions of electronic medical record system utilization : A cross-sectional study in Mafeteng district, Lesotho". The study investigates healthcare workers’ (HCWs) perceptions of EMR systems in the Mafeteng district of Lesotho, adopting the Technology Acceptance Model (TAM) to explore the factors influencing perceived usefulness (PU) and perceived ease of use (PEU). Given the ongoing efforts to implement EMR systems across the healthcare settings in Lesotho, understanding the attitudes and experiences of frontline users is essential for the success of these initiatives