This Excel worksheet contains tables detailing the individual thermotolerance as a response to ambient temperature ramps, flight efficiency, and colony level thermoregulatory ability of the South African (savanna) honey bee Apis mellifera scutellata under both control and treatment (sublethal dose exposure of clothianidin (CLO), imidacloprid (IMI) or thiamethoxam (THX) during experiments)conditions. Data are arranged according to individual thermoregulation (LD50 at temperatures of 46°C to 56°C), flight efficiency (flight mill readings at 25°C, 30°C and 35°C), and colony level thermoregualtion (temperature fluctuations in hive environment over time).