Human serum/plasma miScript miRNA PCR array: profiling circulating microRNAs in pregnancies
<div>To identify
differentially expressed miRNAs, serum miRNAs were profiled using the Human
serum/plasma miScript miRNA PCR array (n=4 per group). The expression of
miR-19b-3p was 6-fold (p=0.033) lower in women with GDM compared to controls,
miR-20a-5p was 4.5-fold (p=0.047) lower in women with T1DM compared to controls
and the expression of miR-29a-3p was 1.8-fold (p=0.002) higher in women with
T2DM compared to controls. Differential expression of additional nine miRNAs,
although not statistically significant (miR-155-5p,
miR-222-3p, miR-9-5p, miR-124-3p, miR-126-3p, miR-210-3p, miR-30d-5p,
miR-27a-3p and miR-128-3p) was demonstrated.
These differentially expressed miRNAs will be measured in serum and urine
samples using SYBR green individual primer assays<br></div>
Funding
South African Medical Research Council (SAMRC), SAMRC Division of Research and Capacity Development, the National Research Foundation (NRF) (grant no. 120832) and the University of Pretoria postgraduate bursary
History
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