University of Pretoria
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miScript_miRNA_PCR_Array_Data_Analysis_050221.xls (137.5 kB)

Human serum/plasma miScript miRNA PCR array: profiling circulating microRNAs in pregnancies

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posted on 2022-02-14, 14:27 authored by Matladi MaseteMatladi Masete
To identify differentially expressed miRNAs, serum miRNAs were profiled using the Human serum/plasma miScript miRNA PCR array (n=4 per group). The expression of miR-19b-3p was 6-fold (p=0.033) lower in women with GDM compared to controls, miR-20a-5p was 4.5-fold (p=0.047) lower in women with T1DM compared to controls and the expression of miR-29a-3p was 1.8-fold (p=0.002) higher in women with T2DM compared to controls. Differential expression of additional nine miRNAs, although not statistically significant (miR-155-5p, miR-222-3p, miR-9-5p, miR-124-3p, miR-126-3p, miR-210-3p, miR-30d-5p, miR-27a-3p and miR-128-3p) was demonstrated. These differentially expressed miRNAs will be measured in serum and urine samples using SYBR green individual primer assays

Funding

South African Medical Research Council (SAMRC), SAMRC Division of Research and Capacity Development, the National Research Foundation (NRF) (grant no. 120832) and the University of Pretoria postgraduate bursary

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Reproductive Biology

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