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Investigating the effects of anti-pseudomonal agents

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posted on 2023-08-17, 09:02 authored by Lebogang SekaloLebogang Sekalo

This dataset presents the investigating of the effects of six primary anti-pseudomonal antibiotics, namely amikacin, cefepime, ciprofloxacin, meropenem, piperacillin and tazobactam, on the planktonic growth of, and formation of biofilm by three different strains of the resilient respiratory pathogen, Pseudomonas aeruginosa [two drug-sensitive strains: the wild-type reference strain, PAO1(WT), and a clinical isolate (DS), as well as a multidrug resistant (MDR) clinically isolated variant of the pathogen].

These agents were investigated individually and in combination with the macrolide antibiotic, clarithromycin. Although all three test strains of P. aeruginosa are resistant to clarithromycin, this agent was included as an adjunct to the conventional anti-pseudomonal agents because of its inhibitory effects on various virulence factors of the pathogen. Following acquisition of the minimal inhibitory concentrations (MICs) of the individual anti-pseudomonal agents with respect to planktonic growth of, and biofilm formation by all three strains of P. aeruginosa, these experiments were repeated using the test anti-pseudomonal antibiotics in combination with clarithromycin. The results showed that amikacin, cefepime, ciprofloxacin and meropenem individually were potent inhibitors of the growth and formation of biofilm of the two susceptible strains of the P. aeruginosa, while, as expected, the MDR strain was highly resistant. When used in combination with clarithromycin, however, synergistic interactions with amikacin, cefepime and ciprofloxacin were observed, while additive activity was observed with the MDR strain.


Funding

National Health Laboratory Service (NHLS)

History

Department/Unit

Immunology

Sustainable Development Goals

  • 3 Good Health and Well-Being