University of Pretoria
Browse

File(s) under embargo

Reason: Waiting for publication

1

year(s)

4

month(s)

8

day(s)

until file(s) become available

Polymerase chain reaction restriction fragment length polymorphism results for self-fertile crosses and single nucleotide polymorphismoutput for hybrid genomes

dataset
posted on 2023-11-01, 10:38 authored by Daniella KrämerDaniella Krämer

A Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) protocol was developed to identify outcrossing between two different species of the fungus Ceratocystis. A unique restriction enzyme cut site in a PCR amplicon produces a banding pattern specific to one of the three fungi and so the presence of two banding patterns indicates the presence of DNA from both of the fungi in the cross. The results of the agarose gels used to visualize the banding patterns are presented in this dataset.

A total of 69 hybrid genomes from crosses between Ceratocystis fimbriata and C. manginecans were analyzed. The inheritance of SNVs across the whole genome was investigated at a fine-scale level. A custom python script produced an output assigning the SNVs identified to the specific parent it was inherited from for analysis of genome structure.

History

Department/Unit

Biochemistry, Genetics and Microbiology

Sustainable Development Goals

  • Not Applicable