Dataset underpinned a thesis titled "Developing pet infection imaging agent for visualizing bacterial peptidoglycan assembly and an article "Evaluation of [68Ga]Ga-DOTA-AeK as a potential imaging tool for PET imaging of cell wall synthesis in bacterial infections". The findings revealed that most radiotracers demonstrated high non-target background signals, which might limit their specificity and sensitivity for diagnostic imaging of bacterial infections. Therefore, this warrants further research and development of target-specific radiotracers for infection imaging.