<p dir="ltr">This study investigated strategies to improve the genetic transformation and regeneration efficiency of <i>Eucalyptus</i>, a species known for its recalcitrance to transformation. The research evaluated the effects of the plant chemical inducer fipexide and <i>Agrobacterium</i>-derived oncogenes on callus induction and de novo meristem formation in wounded <i>Eucalyptus</i> tissues. Comparative analyses of leaf and stem explants were conducted to determine suitable explant sources for regeneration. The findings showed that fipexide enhances callus formation but suppresses shoot regeneration while oncogenes promote callusing yet inhibit shoot elongation. Additionally, induced hairy roots were found capable of forming callus and initiating shoot buds that failed to elongate. Overall, the study provides insights into hormonal and genetic factors influencing <i>Eucalyptus</i> regeneration, contributing to the development of more effective transformation systems for this economically important genus</p>
Funding
Sappi Forest
Department of Science and Technology (DST)
Technology Innovation Agency (TIA)
Technology and Human Resources for Industry Programme (THRIP)