Role of MyD88 intermediate domain in Myddosome complex assembly

The dataset provided contains the tables and figures for the MSc dissertation titled "The role of the MyD88 intermediate domain in the formation of the Myddosome assemble in innate immunity". This project set out to investigate the role of the MyD88 intermediate domain in the assembly of the Myddosome complex. Two variants of MyD88 were therefore expressed in addition to the IRAK-4 death domain to reconstitute the Myddosome complex. The two variants were the MyD88 death domain and MyD88 that contained both the death and intermediate domains. MyD88 that lacked the intermediate domain was found to be an oligomer using size exclusion chromatography and dynamic light scattering. However, MyD88 that contained only the death domain was monomeric.

MyD88 that contained both the death and intermediate domain was mixed with IRAK-4 death domain to reconstitute the Myddosome complex. As expected, we managed to form the Myddosome complex. The presence of this complex was confirmed by size exclusion chromatography and SDS-PAGE. On the contrary, the MyD88 death domain that lacked the intermediate domain failed to form the Myddosome complex when mixed with IRAK-4 death domain. This finding showed that the MyD88 intermediate domain was essential for the Myddosome assembly. It was also in support of existing literature that MyD88 does not interact with IRAK-4 in the absence of its intermediate domain.