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Figure 3-1 Cell density (30% U87-CM).jpg (91.97 kB)

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Figure 3-2 Cell density (40% U87-CM).jpg (103.06 kB)

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Figure 3-3 Cell density (30% 20 U87-CM).jpg (102.72 kB)

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Figure 3-4 (40% 20 U87-CM).jpg (105.65 kB)

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Figure 3-5 xCELLigence (bEnd.3_U87-CM).jpg (104.26 kB)

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Figure 3-6 Cell Growth (bEnd.3_U87-CM).jpg (56.16 kB)

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Figure 3-7 xCELLigence (bEnd.3_Co-culture).jpg (63.85 kB)

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Figure 3-8 Cell Growth (bEnd.3_Co-culture).jpg (39.75 kB)

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Figure 3-9 Migration quantitative data.jpg (25.5 kB)

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Figure 3-10 Scratch Migration.jpg (345.27 kB)

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Figure 3-11 Permeability (U87-CM).jpg (97.66 kB)

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Figure 3-12 Permeability (Co-culture).jpg (61.37 kB)

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Figure 3-13 Protein expression (ZO-1_U87-CM).jpg (90.1 kB)

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Figure 3-14 Protein expression (ZO-1_Co-culture).jpg (88.42 kB)

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Figure 3-15 Protein expression (claudin-5_U87-CM).jpg (96.79 kB)

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Figure 3-16 Protein expression (claudin-5_Co-culture).jpg (93.54 kB)

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Figure 3-17 Protein expression (occludin_U87-CM).jpg (91.54 kB)

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Figure 3-18 Protein expression (occludin_Co-culture).jpg (95.14 kB)

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Effects of glioblastoma cells and conditioned media on brain endothelial cells in vitro

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posted on 2024-02-02, 13:35 authored by Xolisile MokoenaXolisile Mokoena

Brain endothelial (bEnd.3) cells were used as a model of the BBB, co-cultured with glioblastoma (U87) cells or treated with conditioned media from U87 cells. The cell density of mono-cultured brain endothelial cells treated with glioblastoma conditioned media was evaluated through the sulforhodamine-B assay. The effect of U87 cells and conditioned media (CM) on the growth and migration of bEnd.3 cells was assessed using xCELLigence real-time cell analyzer and scratch assay, respectively. The barrier integrity of bEnd.3 cells were evaluated through the sodium fluorescein. The expression of the tight junction (TJ) proteins namely ZO-1, claudin-5, and occludin (presence or absence, size, and modification or degradation states) in brain endothelial cells treated with conditioned media and co-cultured with U87 cells were evaluated using the Western Blot assay.