Producing linear pFastBac plasmid for use in In-Fusion cloning and an example of western blot produced by non-optimal conditions

Figure S1, show the results of double restriction enzyme digestion of the plasmid pFB-NS4 to excise NS4 in order to obtain linearised pFB that could be used in downstream applications such as In-Fusion cloning. Figure S2, shows the results of confirming recombinant protein expression via western blotting using the baculovirus expression system in Sf9 cells. These blots show the amount of protein degradation present from unoptimized blotting conditions to contrast the results obtained from optimized conditions shown in the main dissertation.