University of Pretoria
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Metabolic profiling of industrial Pb-removing microbes

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Version 2 2022-03-16, 13:01
Version 1 2021-07-01, 09:03
journal contribution
posted on 2022-03-16, 13:01 authored by Carla Cilliers, Evans Chirwa, Hendrik Gideon BrinkHendrik Gideon Brink
Data set linked to published journal article.
The objective of the study was to gather insight into the metabolism of lead-removing microorganisms, coupled with Pb(II) removal, biomass viability and nitrate concentrations for Pb(II) bioremoval using an industrially obtained microbial consortium. The consortium used for study has proven to be highly effective at removing aqueous Pb(II) from solution. Anaerobic batch experiments were conducted with Luria-Bertani broth as rich growth medium over a period of 33 h, comparing a lower concentration of Pb(II) with a higher concentration at two different nutrient concentrations. Metabolite profiling and quantification were conducted with the aid of both liquid chromatography coupled with tandem mass spectroscopy (UPLC-HDMS) in a “non-targeted” fashion and high-performance liquid chromatography (HPLC) in a “targeted” fashion. Four main compounds were identified, and a metabolic study was conducted on each to establish their possible significance for Pb(II) bioremoval. The study investigates the first metabolic profile to date for Pb(II) bioremoval, which in turn can result in a clarified understanding for development on an industrial and microbial level


The National Research Foundation (NRF) of South Africa, grant number 106938

The National Research Foundation (NRF) of South Africa, grant number 121891

The National Research Foundation (NRF) of South Africa, grant number 128088



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