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Phylogenetic analyses used for the description of five novel Curvularia species (Pleosporaceae, Pleosporales) isolated from fairy circles in the Namib desert

journal contribution
posted on 2024-05-16, 07:43 authored by Cobus VisagieCobus Visagie, Nicole van Vuuren, Neriman Yilmaz, Michael J. Wingfield

DNA sequence datasets, alignments and phylogenetic trees used in a publication to describe five novel Curvularia species (Pleosporaceae, Pleosporales) isolated from fairy circles in the Namib desert.

For phlogenetic analyses, a reference sequence database was compiled based on recent literature (Crous et al. 2020; Ferdinandez et al. 2021; Iturrieta-González et al. 2020; Kiss et al. 2020; Manamgoda et al. 2012; Marin-Felix et al. 2020). Exserohilum turcicum (CBS 690.71T) and Bipolaris zeae (BRIP 11512IsoPT) were included as outgroups. Sequences were aligned in Geneious Prime® 2023.2.1 using the MAFFT v 7.450 plugin selecting the L-INS-i algorithm (Katoh and Standley 2013), and then manually trimming and adjusting where appropriate. The datasets were partitioned to take into account gene regions, as well as introns and exons. For multi-gene phylogenies, alignments were concatenated in Geneious Prime. Maximum likelihood trees were calculated in IQtree v 2.1.3 (Nguyen et al. 2015) and support in nodes calculated using a bootstrap analysis with 1000 replicates. Phylogenetic trees were visualized using TreeViewer v 2.2.0 and visually edited using Affinity Designer v 2.3.1 (Serif (Europe) Ltd, Nottingham, UK).

Funding

Future Leaders African Independent Research Fellowship Programme (FLAIR), Grant No: FLR\R1\201831

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Forestry and Agricultural Biotechnology Institute

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  • 15 Life on Land

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    Natural and Agricultural Sciences

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