University of Pretoria
8 files

Pulmonary tuberculosis (PTB) symptomatic individuals, their sociodemographic characteristics, genetic diversity and drug resistance patterns of Mycobacterium tuberculosis strains

posted on 2023-03-06, 08:24 authored by Melese RetaMelese Reta, Nontuthuko Excellent Maningi, P. Bernard Fourie

This dataset described the entire number of adult holy water sites (HWS) attendees (≥18 years of age) who were screened for Pulmonary Tuberculosis (PTB) -suggestive symptoms at nine selected study zones (HWS) found across the study region. During the study period, a total of 10,313 adult HWS (≥ 18 years of age) were screened, and 560 individuals were PTB symptomatic and participated. Out of 560 PTB symptomatic HWS attendees who participated, 122 (21.8%) were Lowenstein- Jensen (LJ) culture-positive (bacteriologically confirmed cases). Their sociodemographic characteristics and PTB infection risk factors are detailed. All LJ culture-positive isolates were subjected to spoligotyping, resulting in 116 isolates with interpretable spoligo-patterns. We performed drug susceptibility testing for all 122 (LJ culture)-positive isolates using MTBDRplus v2 and MTBDRsl v2 line probe assays.  

Drug-susceptibility testing (DST) results for first and second line anti-TB drugs are detailed in this dataset. Furthermore, a prospective follow-up study on 438 PTB-symptomatic individuals with culture-negative test results was done to determine the prevalence of developing active TB disease subsequent to residing at a HWS. The duration of follow-up was 12 months, starting on the date that a negative culture result was obtained. Thus, 30 (6.8%) were infected with TB and developed active TB disease post-exposure to HWS. In general, in these attached datasets, details of the study participants, LJ culture-testing results, DST results, as well as genotyping findings of the isolates are illustrated.



Medical Microbiology