Reconsideration of species boundaries and proposed DNA barcodes for Calonectria

Fig.1 is the Phylogenetic tree of the genus Calonectria based on maximum likelihood (ML) analyses of combined DNA dataset of act, cmdA, his3, ITS, LSU, rpb2, tef1 and tub2 gene sequences. Codes A1 to A169 showed the 169 Calonectria species before their taxonomic reconsideration in this study, codes B1 to B120 indicated the 120 accepted Calonectria species after the revision emerging from this study. Seventy-six isolates unable to obtain in this study were marked in blue, their sequences were downloaded from NCBI. 

Fig.2 and Fig.3 are the novel species descibed in the study.

Fig.4 and Fig.5 are the identification success rate (ISR) was calculated for each of the eight putative barcodes for all 120 species of Calonectria and also for species residing in each of the 11 well defined species complexes. The ISR was calculated by dividing the number of identified species for each gene region by the total number of species of Calonectria recognised in the phylogenetic analyses of eight gene regions. The ISR of each candidate DNA barcode was computed based on the eight individual gene phylogenetic trees and the eight-gene combined phylogenetic tree. For each candidate DNA barcode, the ISRs were calculated both for the entire genus Calonectria, as well for each of the recognised species complexes.